Y-1細胞

數(shù)量： 大量

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 小鼠

器官來源： 其他

細胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

品系： LAF1

組織來源： cortex

ATCC Number： CCL-79?

Y-1細胞Designations: Y-1

Depositors: G Sato

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Organ: adrenal gland; tumor

Strain: LAF1

Tissue: cortex

Cellular Products: steroid hormones

Permits/Forms: Y-1細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: detection of LT toxin [21447]

detection of cholera toxin [21556]

detection of verotoxin

Virus Susceptibility: Herpes simplex virus

Pseudorabies virus

Vaccinia virus

Vesicular stomatitis virus

Human poliovirus 1

Y-1細胞Cytogenetic Analysis: modal number = 41; range = 40 to 108. Stemline number is hyperdiploid. Karyotype stable within stemline number. Thirty-nine chromosomes with terminal centromeres plus 2 minute chromosomes; some cells with only l minute chromosome. The first chromosome is quite large and some of the smaller chromosomes are smaller than normal, but no specific structural abnormalities were detected.

Gender: male

Comments: Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: At least 2 times per week (or else hormone production will decrease)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature:Y-1細胞 liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040

References: 1216: Yasumura Y, et al. Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype. Cancer Res. 26: 529-535, 1966. PubMed: 5930699

21447: American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

21556: U.S. Food & Drug AdministrationDetection of cholera enterotoxin (CT) and cytotoxinIn: U.S. Food & Drug AdministrationBacteriological analytical manual8th ed.Gaithersburg, MDAOAC Internationalpp. 9.12-9.15, 1995

23251: Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682

26234: Cohen AI, et al. Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats. Am. J. Pathol. 33: 631-651, 1957. PubMed: 13444454

26235: Cohen AI, et al. In vitro response of functional experimental adrenal tumors to corticotropin ACTH. Proc. Soc. Exp. Biol. Med. 95: 304-309, 1957. PubMed: 13441719

16173349: Rainey WE, et al. Adrenocortical cell lines. Mol. Cell Endocrinol. 228(1-2): 23-38, 2004. PubMed: 15541570