G26.4.1C3/86 [RAT 1C3/86]細胞
運輸方式： 凍存運輸
生長狀態： 懸浮生長
數量： 大量
細胞形態： **樣
**類型： IgG1
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-9893?
G26.4.1C3/86 [RAT 1C3/86]細胞Designations: G26.4.1C3/86 [RAT 1C3/86]
Depositors: Agen Biomedical, Ltd.
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: G26.4.1C3/86 [RAT 1C3/86]細胞lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human red blood cell (erythrocyte, RBC) antigen, glycophorin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with human erythrocytes.
Spleen cells were fused with mouse myeloma cells.
The antibody will bind to, but not agglutinate, freshly collected human red blood cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
G26.4.1C3/86 [RAT 1C3/86]細胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 3947: Rylatt DB, et al. Erythrocyte agglutination assay. G26.4.1C3/86 [RAT 1C3/86]細胞US Patent 4,894,347 dated Jan 16 1990
