13C4細胞
細胞形態： **樣
**類型： IgG1; kappa light chain
數量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： CRL-1794?
生長狀態： 懸浮生長
運輸方式： 凍存運輸
Designations: 13C4
13C4細胞Depositors: AD O'Brien
Isotype: IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against shiga toxin and shiga like toxin I (SLTI)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 13C4細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with purified shiga-like toxin from E. coli H30.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the beta subunit of shiga toxin and SLTI and neutralizes Shiga toxin activities from Shigella dysenteriae type I and Shigella flexneri and Shiga-like toxin activities from Escherichia coli.
Vibrio cholerae, Vibrio parahaemolyticus and Salmonella typhimurium.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
13C4細胞1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 1324: Strockbine NA, et al. 13C4細胞Characterization of monoclonal antibodies against shiga-like toxin from Escherichia coli. Infect. Immun. 50: 695-700, 1985. PubMed: 3905611
