G-8細胞

生長狀態(tài)： 貼壁生長

組織來源： skeletal muscle

細胞形態(tài)： 成肌細胞

年限： fetus fetus

運輸方式： 凍存運輸

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

G-8細胞物種來源： 小鼠

ATCC Number： CRL-1456?

數(shù)量： 大量

Designations: G-8

Depositors: J Peacock

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

G-8細胞Morphology: myoblast

Source: Tissue: skeletal muscle

Cell Type: myoblast myoblast;

Cellular Products: myosin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: acetylcholine (The cells are highly responsive to acetylcholine.)

Tumorigenic: No

G-8細胞Cytogenetic Analysis: modal number = 66

Age: fetus fetus

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks.Differentiation is enhanced by reducing the sera to 2% each.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Doubling Time: 25 to 27 hrs

References: 1063: Christian CN, et al. G-8細胞Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191