1D4細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-8068?
數量： 大量
生長狀態： 懸浮生長
細胞形態： **樣
**類型： IgG1
運輸方式： 凍存運輸
Designations: 1D4
1D4細胞Depositors: The United States of America
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against nucleocapsid (p40) of herpes simplex virus type 1 (HSV-1)
Permits/Forms: 1D4細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: The original deposit of this cell line was contaminated with microorganisms. The line was treated with antibiotics and is now cured of the contamination. Animals were immunized with purified HSV-1 nucleocapsids.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody precipitates p40 and p45 of HSV-1 but not HSV-2.
The supernatant may have to be concentrated for use.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 1 mM sodium pyruvate and antibiotics, 15%; fetal bovine serum, 15%
Subculturing: 1D4細胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 70%; fetal bovine serum, 20%; DMSO, 10%
References: 1004: Zweig M, et al. Production of monoclonal antibodies against nucleocapsid proteins of Herpes simplex Virus types 1 and 2. J. Virol. 32: 676-678, 1979. PubMed: 91687
3510: Zweig M, et al. 1D4細胞Test methods employing monoclonal antibodies against Herpes simplex virus types 1 and 2 nucleocapsids proteins. US Patent 4,430,437 dated Feb 7 1984
