Le Ana細胞

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-1192?

相關(guān)**： 馬凡氏綜合征

數(shù)量： 大量

器官來源： 皮膚

年限： 2 years

運輸方式： 凍存運輸

細胞形態(tài)： 成纖維樣

是否是腫瘤細胞： 0

Le Ana細胞物種來源： 人

Designations: Le Ana

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: Le Ana細胞Marfan syndrome

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Age: 2 years

Gender: female

Comments: The tissue donor suffered from an atypical form of Marfan syndrome.

The patient presented with no eye findings, normal height and severe joint laxity.

Propagation: Le Ana細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Le Ana細胞Subculture every 7 to 9 days.