184A1細胞

細胞類型： 其他細胞類型

年限： 21 years

是否是腫瘤細胞： 0

物種來源： 人

器官來源： **

組織來源： epithelium

細胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-8798?

184A1細胞運輸方式： 凍存運輸

相關(guān)**： 正常

數(shù)量： 大量

Designations: 184A1

Depositors: The United States of America

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Tissue: 184A1細胞epithelium

Disease: normal

Cell Type: chemically transformed

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11

D16S539: 11,12

D5S818: 11,13

D7S820: 9,11

THO1: 9.3

TPOX: 11

vWA: 18,19

Cytogenetic Analysis: 45, XX

Age: 21 years

Gender: 184A1細胞female

Comments: The 184A1 cell line was established from normal mammarytissue obtained from a normal reduction mammoplasty.

Cells derived from the tissue were exposed to benzo(a)pyrene, and a transformed line was established.

The line appears to be immortal, but is not malignant.

When seeded at low density, the cells grow as single cells.

Propagation: ATCC complete growth medium: The base medium for this cell line (MEBM) along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit. To make the complete growth medium, you will need to add the following components to the kit (sold separately):

0.005 mg/ml transferrin

1 ng/ml cholera toxin

Note: Do not filter complete medium

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: 184A1細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to10 minutes.Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

Place culture vessels in incubators at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Culture medium, 75%; fetal bovine serum, 15%; glycerol, 10%

References: 21894: . Transformation of human epithelial cells. Boca Raton, FL: CRC Press; 1992.

21926: Stampfer MR. Continuous human cell lines and method of making same. US Patent 4,808,532 dated Feb 28 1989

22413: Walen KH, Stampfer MR. Chromosome analyses of human mammary epithelial cells at stages of chemical-induced transformation progression to immortality. Cancer Genet. Cytogenet. 37: 249-261, 1989. PubMed: 2702624

23289: Stampfer MR, Bartley JC.184A1細胞 Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo[a]pyrene. Proc. Natl. Acad. Sci. USA 82: 2394-2398, 1985. PubMed: 3857588