LCL 8664細胞
細胞類型： B**細胞
ATCC Number： CRL-1805?
相關**： **瘤
是否是腫瘤細胞： 0
物種來源： 獼猴
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
年限： 5 years
數量： 大量
LCL 8664細胞細胞形態(tài)： **樣
Designations: LCL 8664
Depositors: SR Rangan
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Macaca mulatta
Morphology: lymphoblast
Source: Disease: lymphoma
Cell Type: LCL 8664細胞B lymphocyte;
Cellular Products: B lymphotropic herpesvirus
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Age: 5 years
Gender: male
Comments: CAUTION: the cells are infected with and produce a B lymphotropic herpesvirus (RhEBV) and may represent a potential biohazard.
Free RhEBV is present in the supernatant, and rhesus B lymphocyte transforming activity is present in the medium.
About 1% of the cells express virus capsid antigen, and all express the virus related nuclear antigen.
Propagation: LCL 8664細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp5 cells/ml and maintain between 4 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22860: Rangan SR, et al. Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma. Int. J. Cancer 38: 425-432, 1986.LCL 8664細胞 PubMed: 3017870
