RL細胞
細胞類型： B**細胞
生長狀態： 懸浮生長
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： CRL-2261?
相關**： 其他**
運輸方式： 凍存運輸
數量： 大量
年限： 52 years
細胞形態： **樣
**類型： IgM; lambda light chain
組織來源： ascites
Designations: RL
RL細胞Depositors: M Beckwith
Isotype: IgM; lambda light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Tissue: ascites
Disease: non-Hodgkin's lymphoma
Cell Type: B lymphoblast;
Cellular Products: immunoglobulin; surface IgM and IgD; secreted IgM (low levels)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. RL細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 8,11
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9,10
TPOX: 9,11
vWA: 19,20
Cytogenetic Analysis: the cells possess a 14:18 translocation
Age: 52 years
Gender: male
Ethnicity: Caucasian
Comments: RL細胞RL is a human non-Hodgkin's lymphoma B cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
Exposure of RL cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
RL cells have been reported to be Epstein-Barr virus genome negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. RL細胞Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
